*Prices as of July 20, 2023. Pricing is subject to change. Please visit DUGSIM for up-to-date pricing.
We are a cost-recovery shared resource and do not make a profit on our services per Duke policy. Our price includes our facility fee for our services and the cost of consumables. Per Duke policy, there are no discounts on services.
DNA-Sequencing Illumina Library Building Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
DNA-Seq with KAPA HyperPrep -- 1 sample |
LP-DNA-004
|
$85 |
$100 |
|
Tagmentation library build with Illumina DNA Prep 1/8 Rxn up to 16 samples |
LP-DNA-009
|
$539 |
$657 |
|
Tagmentation library build with Illumina DNA Prep Full Rxn -- 1 sample |
LP-DNA-009-B |
$61 |
$69 |
|
IDT Human Exome up to 12 samples |
LP-CAP-013 |
$1,380 |
$1,615 |
|
Twist - Mouse whole exome - up to 16 samples |
LP-CAP-015 |
$1,610.00 |
$1,840.58 |
|
Methyl-Seq per sample |
LP-MET-002 |
$317.00 |
$434.12 |
|
Twist Methylome up to 8 samples |
LP-CAP_030 |
$1,615.00 |
$1,732.12 |
|
ChIP-seq/Amplicon Library Preparation with KAPA HyperPrep - 1 sample |
LP-DNA-008 |
$73 |
$88 |
|
Custom Capture Panels |
Contact us |
|
|
RNA-Sequencing Illumina Library Building Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus -- 1 sample |
LP-RNA-003
|
$129 |
$144 |
| Stranded mRNA-Seq HyperPrep -- 1 sample | LP-RNA-004 | $91 | $105.64 |
| Qiagen UPXome Ultra Low Input RNA-Seq -- 1 sample | LP-RNA-012 | $139 | $154 |
|
NuGEN mRNA-Seq with AnyDeplete Globin -- 1 sample |
LP-RNA-016
|
$152 |
$167 |
|
smRNA-seq -- 1 sample |
LP-RNA-019 |
$152 |
$167 |
|
COVID-Seq, 94 samples + 2 controls |
LP-RNA-020 |
$3454.00 |
$3571.12 |
|
TruSeq RNA Exome for up to 3 FFPE or degraded samples |
LP-RNA-014 |
$1471.00 |
$1932.16 |
Illumina Sequencing Services
| Platform | Flow Cell | Read Length** | Code | Duke Price | Non-Duke Price |
|---|---|---|---|---|---|
| MiSeq | v2 | 2 x 25 | I-S-100 | $1,328 | $1,551 |
| v2 | 1 x 50 | I-S-110 | $1,328 | $1,551 | |
| v2 | 2 x 150 | I-S-120 | $1,595 | $1,821 | |
| v2 | 2 x 250 | I-S-130 | $1,752 | $1,975 | |
| v2 Nano | 2 x 150 | I-S-150 | $708 | $931 | |
| v2 Nano | 2 x 250 | I-S-170 | $777 | $1,000 | |
| v2 Micro | 2 x 150 | I-S-180 | $883 | $1,106 | |
| v3 | 2 x 75 | I-S-160 | $1,434 | $1,657 | |
| v3 | 2 x 300 | I-S-140 | $2,170 | $2,393 | |
| NovaSeq 6000 | S-Prime Full Flow Cell | 2 x 50 | I-S-7020 | $3,040 | $3,421 |
| S-Prime Full Flow Cell | 2 x 100 | I-S-7030 | $3,789 | $4,170 | |
| S-Prime Full Flow Cell | 2 x 150 | I-S-7010 | $4,077 | $4,458 | |
| S-Prime Full Flow Cell | 2 x 250 | I-S-7000 | $5,458 | $5,839 | |
| S-Prime Lane | 2 x 50 | I-S-7025 | $1,692 | $1,882 | |
| S-Prime Lane | 2 x 100 | I-S-7030-2 | $2,067 | $2,257 | |
| S-Prime Lane | 2 x 150 | I-S-7015 | $2,212 | $2,402 | |
| S-Prime Lane | 2 x 250 | I-S-7000-2 | $2,905 | $3,095 | |
| S1 Full Flow Cell | 2 x 50 | I-S-6040 | $5,225 | $5,606 | |
| S1 Full Flow Cell | 2 x 100 | I-S-6050 | $6,206 | $6,587 | |
| S1 Full Flow Cell | 2 x 150 | I-S-6060 | $6,667 | $7,048 | |
| S1 Lane | 2 x 50 | I-S-6070 | $2,785 | $2,975 | |
| S1 Lane | 2 x 100 | I-S-6080 | $3,276 | $3,466 | |
| S1 Lane | 2 x 150 | I-S-6090 | $3,506 | $3,696 | |
| S2 Full Flow Cell | 2 x 50 | I-S-6000 | $9,911 | $10,866 | |
| S2 Full Flow Cell | 2 x 100 | I-S-6020 | $11,962 | $12,881 | |
| S2 Full Flow Cell | 2 x 150 | I-S-6010 | $12,617 | $13,572 | |
| S2 Lane | 2 x 50 | I-S-6000-2 | $5,128 | $5,605 | |
| S2 Lane | 2 x 100 | I-S-6020-2 | $6,135 | $6,612 | |
| S2 Lane | 2 x 150 | I-S-6010-2 | $6,481 | $6,7958 | |
| S4 Full Flow Cell | 2 x 100 | I-S-6100 | $16,310 | $17,183 | |
| S4 Full Flow Cell | 2 x 150 | I-S-6030 | $18,010 | $18,883 | |
| S4 Full Flow Cell | 2 x 35 | I-S-6035 | $12,832 | $13,705 | |
| S4 Lane | 2 x 100 | I-S-6200 | $4,250 | $4,468 | |
| S4 Lane | 2 x 150 | I-S-6001 | $4,669 | $4,887 | |
| NextSeq 1000 | P1 | 2 x 50 | I-S-1000-1 | $1,282 | $1,522 |
| P1 | 2 x 100 | I-S-1000-2 | $1,628 | $1,868 | |
| P1 | 2 x 150 | I-S-1000-3 | $1,628 | $1,868 | |
| P1 | 2 x 300 | I-S-1000-4 | $2,270 | $2,510 | |
| P2 | 2 x 50 | I-S-1000-5 | $1,830 | $2,070 | |
| P2 | 2 x 100 | I-S-1000-6 | $3,095 | $3,335 | |
| P2 | 2 x 150 | I-S-1000-7 | $3,967 | $4,216 | |
| P2 | 2 x 300 | I-S-1000-8 | $4,295 | $4,535 | |
| NovaSeq X Plus | 10B | 2 x 50 | I-S-X-10B-100 | $8,900 | $9,117 |
| 10B | 2 x 100 | I-S-X-10B-200 | $11,000 | $11,217 | |
| 10B | 2 x 150 | I-S-X-10B-300 | $11,700 | $11,917 | |
| 10B Lane | Any Configuration*** | I-S-X-10B-Lane | $1,465 | $1,492 |
**default sequencing protocol; we can run custom sequencing protocols with purchase of full flow cell
***We can run custom sequencing protocols on NovaSeq X Plus 10B lanes
Sequencing with Oxford Nanopore Technologies & Tapestri
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
ONT GridION library preparation and sequencing - 1 sample |
ONP-G-01
|
$1,726 |
$2,007 |
|
ONT GridION barcoded library prep and sequencing (up to 8 samples) |
ONP-G-02
|
$2,497 |
$2,748 |
|
ONT GridION Extra Flow Cell |
ONP-S-01 |
$1,214 |
$1,339 |
|
Other ONT applications |
Contact us |
|
|
|
Tapestri single cell library preparation |
TL-000 |
$246.00 |
$363.12 |
Extraction and Other Wet-Lab Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
RNA extraction from PAXgene Blood Tube) |
SE-001 |
$46 |
$59 |
|
RNA extraction from pellets |
SE-004 |
$36 |
$49 |
|
miRNA extraction from serum/plasma |
SE-005 |
$37 |
$50 |
|
DNA extraction from PBMCs, Buffy coat or EDTA blood |
SE-006 |
$37 |
$51.64 |
|
RNA extraction from Nasal Swabs |
SE-007 |
$37 |
$51.64 |
|
Viral RNA extraction from Nasal Swabs (96) |
SE-007-B |
$754 |
$915.04 |
| Library AMPure Bead Clean-up -- 1 sample | L-C-001 | $30 | $44.64 |
|
Pooling of up to 12 libraries for sequencing |
SP-001 |
$183 |
$220 |
| HMW DNA Extraction using Circulomics | SE-008 | $69 | $83.64 |
| Illumina library quantification (qPCR) | SP-006 | $43 | $51 |
While we no longer offer QC only services, the Microbiome Core Facility does.
Data Processing Services***
| Description | Code | Duke Price |
|---|---|---|
| Bioinformatics Free 1 Hour Consultation (>$450 value) | DP-06 | $0 |
| Bioinformatics Free 1 Hour Wrap-up Meeting (>$300 value) | DP-20 |
$0 |
| RNA-Seq Pipeline Cost per Sample (first 6-10 samples) | DP-07 | $65 |
| RNA-Seq Pipeline Cost per Sample (next 10+ samples) | DP-08 | $25 |
| ATAC-Seq/ChIP-Seq Pipeline Cost per Sample (first 2-7 samples) | DP-09 | $110 |
| ATAC-Seq/ChIP-Seq Pipeline Cost per Sample (next 8+ samples) | DP-10 | $40 |
| Whole Genome Pipeline Cost per Sample (first 4 samples) | DP-11 | $130 |
| Whole Genome Pipeline Cost per Sample (next 5+ samples0 | DP-12 | $55 |
| Whole Exome Pipeline Cost per Sample (first 1-4 samples) | DP-13 | $100 |
| Whole Exome Pipeline Cost per Sample (next 5+ samples) | DP-14 | $40 |
| Methyl-Seq Pipeline Cost per Sample (first 2-7 samples) | DP-15 | $110 |
| Methyl-Seq Pipeline Cost per Sample (next 8+ samples) | DP-16 | $40 |
| Mapping and Basic QC Data Processing (first 6-19 samples) | DP-17 | $40 |
| Mapping and Basic QC Data Processing (next 20+ samples) | DP-18 | $20 |
| Hourly Rate | DP-19 | $159 |
| Hourly Rate - 15 minute rate | DP-21 | $39.75 |
***Not available outside of Duke