*Prices as of July 20, 2023. Pricing is subject to change. Please visit SeqLIMS for up-to-date pricing.
We are a cost-recovery shared resource and do not make a profit on our services per Duke policy. Our price includes our facility fee for our services and the cost of consumables. Per Duke policy, there are no discounts on services.
- DNA-Sequencing Illumina Library Building Services
- RNA-Sequencing Illumina Library Building Services
- Illumina Sequencing Services
- Sequencing with Oxford Nanopore Technologies & Tapestri
- Extraction and Other Wet-Lab Services
- Bioinformatics Services
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DNA-Sequencing Illumina Library Building Services
Description |
Code |
Duke Price |
Non-Duke Price |
---|---|---|---|
DNA-Seq with KAPA HyperPrep -- 1 sample |
LP-DNA-004
|
$85 |
$100 |
Tagmentation library build with Illumina DNA Prep 1/8 Rxn up to 16 samples |
LP-DNA-009
|
$539 |
$657 |
Tagmentation library build with Illumina DNA Prep Full Rxn -- 1 sample |
LP-DNA-009-B |
$61 |
$69 |
IDT Human Exome up to 12 samples |
LP-CAP-013 |
$1,380 |
$1,615 |
Twist - Mouse whole exome - up to 16 samples |
LP-CAP-015 |
$1,610.00 |
$1,840.58 |
Methyl-Seq per sample |
LP-MET-002 |
$317.00 |
$434.12 |
Twist Methylome up to 8 samples |
LP-CAP_030 |
$1,615.00 |
$1,732.12 |
ChIP-seq/Amplicon Library Preparation with KAPA HyperPrep - 1 sample |
LP-DNA-008 |
$73 |
$88 |
Custom Capture Panels |
Contact us |
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RNA-Sequencing Illumina Library Building Services
Description |
Code |
Duke Price |
Non-Duke Price |
---|---|---|---|
Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus -- 1 sample |
LP-RNA-003
|
$129 |
$144 |
Stranded mRNA-Seq HyperPrep -- 1 sample | LP-RNA-004 | $91 | $105.64 |
Qiagen UPXome Ultra Low Input RNA-Seq -- 1 sample | LP-RNA-012 | $139 | $154 |
NuGEN mRNA-Seq with AnyDeplete Globin -- 1 sample |
LP-RNA-016
|
$152 |
$167 |
smRNA-seq -- 1 sample |
LP-RNA-019 |
$152 |
$167 |
COVID-Seq, 94 samples + 2 controls |
LP-RNA-020 |
$3454.00 |
$3571.12 |
TruSeq RNA Exome for up to 3 FFPE or degraded samples |
LP-RNA-014 |
$1471.00 |
$1932.16 |
Illumina Sequencing Services
Platform | Flow Cell | Read Length** | Code | Duke Price | Non-Duke Price |
---|---|---|---|---|---|
MiSeq | v2 | 2 x 25 | I-S-100 | $1,328 | $1,551 |
v2 | 1 x 50 | I-S-110 | $1,328 | $1,551 | |
v2 | 2 x 150 | I-S-120 | $1,595 | $1,821 | |
v2 | 2 x 250 | I-S-130 | $1,752 | $1,975 | |
v2 Nano | 2 x 150 | I-S-150 | $708 | $931 | |
v2 Nano | 2 x 250 | I-S-170 | $777 | $1,000 | |
v2 Micro | 2 x 150 | I-S-180 | $883 | $1,106 | |
v3 | 2 x 75 | I-S-160 | $1,434 | $1,657 | |
v3 | 2 x 300 | I-S-140 | $2,170 | $2,393 | |
NovaSeq 6000 | S-Prime Full Flow Cell | 2 x 50 | I-S-7020 | $3,040 | $3,421 |
S-Prime Full Flow Cell | 2 x 100 | I-S-7030 | $3,789 | $4,170 | |
S-Prime Full Flow Cell | 2 x 150 | I-S-7010 | $4,077 | $4,458 | |
S-Prime Full Flow Cell | 2 x 250 | I-S-7000 | $5,458 | $5,839 | |
S-Prime Lane | 2 x 50 | I-S-7025 | $1,692 | $1,882 | |
S-Prime Lane | 2 x 100 | I-S-7030-2 | $2,067 | $2,257 | |
S-Prime Lane | 2 x 150 | I-S-7015 | $2,212 | $2,402 | |
S-Prime Lane | 2 x 250 | I-S-7000-2 | $2,905 | $3,095 | |
S1 Full Flow Cell | 2 x 50 | I-S-6040 | $5,225 | $5,606 | |
S1 Full Flow Cell | 2 x 100 | I-S-6050 | $6,206 | $6,587 | |
S1 Full Flow Cell | 2 x 150 | I-S-6060 | $6,667 | $7,048 | |
S1 Lane | 2 x 50 | I-S-6070 | $2,785 | $2,975 | |
S1 Lane | 2 x 100 | I-S-6080 | $3,276 | $3,466 | |
S1 Lane | 2 x 150 | I-S-6090 | $3,506 | $3,696 | |
S2 Full Flow Cell | 2 x 50 | I-S-6000 | $9,911 | $10,866 | |
S2 Full Flow Cell | 2 x 100 | I-S-6020 | $11,962 | $12,881 | |
S2 Full Flow Cell | 2 x 150 | I-S-6010 | $12,617 | $13,572 | |
S2 Lane | 2 x 50 | I-S-6000-2 | $5,128 | $5,605 | |
S2 Lane | 2 x 100 | I-S-6020-2 | $6,135 | $6,612 | |
S2 Lane | 2 x 150 | I-S-6010-2 | $6,481 | $6,7958 | |
S4 Full Flow Cell | 2 x 100 | I-S-6100 | $16,310 | $17,183 | |
S4 Full Flow Cell | 2 x 150 | I-S-6030 | $18,010 | $18,883 | |
S4 Full Flow Cell | 2 x 35 | I-S-6035 | $12,832 | $13,705 | |
S4 Lane | 2 x 100 | I-S-6200 | $4,250 | $4,468 | |
S4 Lane | 2 x 150 | I-S-6001 | $4,669 | $4,887 | |
NextSeq 1000 | P1 | 2 x 50 | I-S-1000-1 | $1,282 | $1,522 |
P1 | 2 x 100 | I-S-1000-2 | $1,628 | $1,868 | |
P1 | 2 x 150 | I-S-1000-3 | $1,628 | $1,868 | |
P1 | 2 x 300 | I-S-1000-4 | $2,270 | $2,510 | |
P2 | 2 x 50 | I-S-1000-5 | $1,830 | $2,070 | |
P2 | 2 x 100 | I-S-1000-6 | $3,095 | $3,335 | |
P2 | 2 x 150 | I-S-1000-7 | $3,967 | $4,216 | |
P2 | 2 x 300 | I-S-1000-8 | $4,295 | $4,535 | |
NovaSeq X Plus | 10B | 2 x 50 | I-S-X-10B-100 | $8,900 | $9,117 |
10B | 2 x 100 | I-S-X-10B-200 | $11,000 | $11,217 | |
10B | 2 x 150 | I-S-X-10B-300 | $11,700 | $11,917 | |
10B Lane | Any Configuration*** | I-S-X-10B-Lane | $1,465 | $1,492 |
**default sequencing protocol; we can run custom sequencing protocols with purchase of full flow cell
***We can run custom sequencing protocols on NovaSeq X Plus 10B lanes
Sequencing with Oxford Nanopore Technologies & Tapestri
Description |
Code |
Duke Price |
Non-Duke Price |
---|---|---|---|
ONT GridION library preparation and sequencing - 1 sample |
ONP-G-01
|
$1,726 |
$2,007 |
ONT GridION barcoded library prep and sequencing (up to 8 samples) |
ONP-G-02
|
$2,497 |
$2,748 |
ONT GridION Extra Flow Cell |
ONP-S-01 |
$1,214 |
$1,339 |
Other ONT applications |
Contact us |
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Tapestri single cell library preparation |
TL-000 |
$246.00 |
$363.12 |
Extraction and Other Wet-Lab Services
Description |
Code |
Duke Price |
Non-Duke Price |
---|---|---|---|
RNA extraction from PAXgene Blood Tube) |
SE-001 |
$46 |
$59 |
RNA extraction from pellets |
SE-004 |
$36 |
$49 |
miRNA extraction from serum/plasma |
SE-005 |
$37 |
$50 |
DNA extraction from PBMCs, Buffy coat or EDTA blood |
SE-006 |
$37 |
$51.64 |
RNA extraction from Nasal Swabs |
SE-007 |
$37 |
$51.64 |
Viral RNA extraction from Nasal Swabs (96) |
SE-007-B |
$754 |
$915.04 |
Library AMPure Bead Clean-up -- 1 sample | L-C-001 | $30 | $44.64 |
Pooling of up to 12 libraries for sequencing |
SP-001 |
$183 |
$220 |
HMW DNA Extraction using Circulomics | SE-008 | $69 | $83.64 |
Illumina library quantification (qPCR) | SP-006 | $43 | $51 |
While we no longer offer QC only services, the Microbiome Core Facility does.
Data Processing Services***
Description | Code | Duke Price |
---|---|---|
Bioinformatics Free 1 Hour Consultation (>$450 value) | DP-06 | $0 |
Bioinformatics Free 1 Hour Wrap-up Meeting (>$300 value) | DP-20 |
$0 |
RNA-Seq Pipeline Cost per Sample (first 6-10 samples) | DP-07 | $65 |
RNA-Seq Pipeline Cost per Sample (next 10+ samples) | DP-08 | $25 |
ATAC-Seq/ChIP-Seq Pipeline Cost per Sample (first 2-7 samples) | DP-09 | $110 |
ATAC-Seq/ChIP-Seq Pipeline Cost per Sample (next 8+ samples) | DP-10 | $40 |
Whole Genome Pipeline Cost per Sample (first 4 samples) | DP-11 | $130 |
Whole Genome Pipeline Cost per Sample (next 5+ samples0 | DP-12 | $55 |
Whole Exome Pipeline Cost per Sample (first 1-4 samples) | DP-13 | $100 |
Whole Exome Pipeline Cost per Sample (next 5+ samples) | DP-14 | $40 |
Methyl-Seq Pipeline Cost per Sample (first 2-7 samples) | DP-15 | $110 |
Methyl-Seq Pipeline Cost per Sample (next 8+ samples) | DP-16 | $40 |
Mapping and Basic QC Data Processing (first 6-19 samples) | DP-17 | $40 |
Mapping and Basic QC Data Processing (next 20+ samples) | DP-18 | $20 |
Hourly Rate | DP-19 | $159 |
Hourly Rate - 15 minute rate | DP-21 | $39.75 |
***Not available outside of Duke