SGT Pricing

*Prices as of July 30, 2024. Pricing is subject to change. Please visit SeqLIMS for up-to-date pricing. If you don’t see your library preparation of interest, please reach out to us. External academic clients may provide their own reagents for PacBio sequencing and can reach out to us to inquire about pricing and logistics. Potential industry partners can reach out to us to inquire about pricing.

We are a cost-recovery shared resource and do not make a profit on our services per Duke policy. Our price includes our facility fee for our services and the cost of consumables. Per Duke policy, there are no discounts on services.

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DNA-Sequencing Illumina Library Building Services

Description

Code

Duke Price

Non-Duke Price

DNA-Seq library with KAPA HyperPrep per sample
Tier 1: Price/sample 1-47

LP-DNA-Seq

 

$137

$222

DNA-Seq library with KAPA HyperPrep per sample
Tier 2: Price/sample 48-N
LP-DNA-Seq $94 $153

Tagmentation library - full volume reaction for low DNA input
Tier 1: Price/sample 1-47

LP-DNA-TAG-01

 

$81

$105

Tagmentation library - full volume reaction for low DNA input
Tier 2: Price/sample 48-N

LP-DNA-TAG-01

$60

$74

Tagmentation library - 1/4 volume reaction for high DNA input
Tier 1: Price/sample 1-47
LP-DNA-TAG-Low $53 $87
Tagmentation library - 1/4 volume reaction for high DNA input
Tier 2: Price/sample 48-N
LP-DNA-TAG-Low $32 $52

IDT Human Exome (batches of 12 samples)
Tier 1: Price/sample 1-47

LP-cap-hum-ex

$241

$389

IDT Human Exome (batches of 12 samples)
Tier 2: Price/sample 48-N
LP-cap-hum-ex $129 $209
FFPE - DNA-Seq library with KAPA HyperPrep per specimen
Tier 1: Price/sample 1-47
LP-FFPE-DNA-Seq $206 $332
FFPE - DNA-Seq library with KAPA HyperPrep per specimen
Tier 2: Price/sample 48-N
LP-FFPE-DNA-Seq $163 $263

ChIP-seq/amplicon Library Preparation with KAPA HyperPrep per sample
Tier 1: Price/sample 1-47

LP-DNA-ChIP-amp

$86

$140

ChIP-Seq/amplicon library with KAPA HyperPrep per specimen
Tier 2: Price/sample 48-N
LP-DNA-ChIP-amp $65 $79

Custom Capture Panels

Contact us

 

 

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RNA-Sequencing Illumina Library Building Services

Description

Code

Duke Price

Non-Duke Price

mRNA-Seq library with KAPA HyperPrep per sample

LP-RNA-Seq

 

$95

$154

mRNA-Seq library with Human Globin AnyDeplete per sample LP-RNA-Seq-glob $155 $251
Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample LP-RNA-total $151 $189

Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample

LP-RNA-total

 

$143

$170

smRNA-Seq library per sample

LP-RNA-sm-Seq

$156

$252

Takara ultra-low-input RNA-Seq library with KAPA HyperPrep per sample

LP-RNA-Seq-lo-B

$173

$280

FFPE - Total RNA library with Ribo-Zero Plus per sample
Tier 1: Price/sample 1-47

LP-FFPE-RNA-seq

$280

$451

FFPE - Total RNA library with Ribo-Zero Plus per sample
Tier 2: Price/sample 48-N
LP-FFPE-RNA-seq $191 $313

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Illumina Sequencing Services

Platform Flow Cell Read Length** Code Duke Price  Non-Duke Price
MiSeq v2 2 x 25 I-S-100 $1,328 $1,551
  v2 1 x 50 I-S-110 $1,328 $1,551
  v2 2 x 150 I-S-120 $1,595 $1,821
  v2 2 x 250 I-S-130 $1,752 $1,975
  v2 Nano 2 x 150 I-S-150 $708 $931
  v2 Nano 2 x 250 I-S-170 $777 $1,000
  v2 Micro 2 x 150 I-S-180 $883 $1,106
  v3 2 x 75 I-S-160 $1,434 $1,657
  v3 2 x 300 I-S-140 $2,170 $2,393
           
NextSeq 1000 P1 2 x 50 I-S-1000-1 $1,282 $1,522
  P1 2 x 100 I-S-1000-2 $1,628 $1,868
  P1 2 x 150 I-S-1000-3 $1,628 $1,868
  P1 2 x 300 I-S-1000-4 $2,270 $2,510
  P2 2 x 50 I-S-1000-5 $1,830 $2,070
  P2 2 x 100 I-S-1000-6 $3,095 $3,335
  P2 2 x 150 I-S-1000-7 $3,967 $4,216
  P2 2 x 300 I-S-1000-8 $4,295 $4,535
           
NovaSeq X Plus 10B 2 x 50 I-S-X-10B-100 $8,900 $9,117
  10B 2 x 100 I-S-X-10B-200 $11,000 $11,217 
  10B 2 x 150 I-S-X-10B-300 $11,700 $11,917
  10B Lane Any Configuration*** I-S-X-10B-Lane $1,465 $1,492

**default sequencing protocol; we can run custom sequencing protocols with purchase of full flow cell
***We can run custom read length protocols on NovaSeq X Plus 10B lanes. Custom primers must be run on a full flow cell

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PacBio Library Building and Sequencing

Description Code Duke Price Non-Duke Price
HiFi library (large genomes > 200 MB) per sample PB-LP-HiFi $568 $916
HiFi library (small genomes < 200 MB) per sample PB-LP-HiFi-2 $157 $254
Kinnex 16S library per sample PB-LP-Kin-16S $946 $1,524
Kinnex single cell RNA library per sample PB-LP-MAS $946 $1,524
RNA library per sample PB-LP-RNA $683 $1,101
Revio SMRT cell 30 -hour sequencing PB-Rev-SMRT-30 $1,783 $2,396

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Extraction and Other Wet-Lab Services

Description

Code

Duke Price

Non-Duke Price

DNA extraction from PBMCs, buffy coat blood, or EDTA blood per specimen

SP-DNA-blood

$45

$73

HMW DNA extraction per specimen

SP-DNA-HMW

$72

$117

RNA extraction from blood per specimen

SP-RNA-blood

$44

$72

RNA extraction from cell pellet per specimen

SP-RNA-cell

$41

$67

miRNA extraction from serum or plasma per specimen

SP-miRNA-serum

$41

$67

Quantification (qPCR) per library

LP-X-qPCR

$45

$73.50

AMPure bead cleanup per library LP-X-rm-ad-dim $46 $75

Pooling for up to 12 client-made libraries

Pooling

$183

$220

While we no longer offer QC only services, the Microbiome Core Facility does.  

 

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Bioinformatics***

Description Code Duke Price
Free One-Hour Bioinformatics Consultation - over $450 value B-free-consult $0
Free One-Hour Bioinformatics Wrap-Up Meeting - Over $300 value B-free-wrap-up $0
Whole Exome Pipeline per Sample B-exome $414
Whole Genome Pipeline per Sample B-genome

$414

RNA-Seq Pipeline per Sample B-RNA-Seq $624
ATAC-Seq/ChIP-Seq Pipeline per Sample B-ATAC-ChIP-Seq $454
Methyl-Seq Pipeline per Sample

B-Methyl-Seq

$454
Mapping and Basic QC per Sample B-mapping-QC $40
Hourly Rate B-rate-60 $175
Quarter-Hourly Rat B-rate-15 $43.75

SoM CPU charges

B-CPU $0.02

***Not available outside of Duke

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