*Prices as of July 30, 2024. Pricing is subject to change. Please visit SeqLIMS for up-to-date pricing. If you don’t see your library preparation of interest, please reach out to us. External academic clients may provide their own reagents for PacBio sequencing and can reach out to us to inquire about pricing and logistics. Potential industry partners can reach out to us to inquire about pricing.
We are a cost-recovery shared resource and do not make a profit on our services per Duke policy. Our price includes our facility fee for our services and the cost of consumables. Per Duke policy, there are no discounts on services.
- DNA-Sequencing Illumina Library Building Services
- RNA-Sequencing Illumina Library Building Services
- Illumina Sequencing Services
- PacBio Library Building and Sequencing
- Extraction and Other Wet-Lab Services
- Bioinformatics Services
DNA-Sequencing Illumina Library Building Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
DNA-Seq library with KAPA HyperPrep per sample |
LP-DNA-Seq
|
$137 |
$222 |
| DNA-Seq library with KAPA HyperPrep per sample Tier 2: Price/sample 48-N |
LP-DNA-Seq | $94 | $153 |
|
Tagmentation library - full volume reaction for low DNA input |
LP-DNA-TAG-01
|
$81 |
$105 |
|
Tagmentation library - full volume reaction for low DNA input |
LP-DNA-TAG-01 |
$60 |
$74 |
| Tagmentation library - 1/4 volume reaction for high DNA input Tier 1: Price/sample 1-47 |
LP-DNA-TAG-Low | $53 | $87 |
| Tagmentation library - 1/4 volume reaction for high DNA input Tier 2: Price/sample 48-N |
LP-DNA-TAG-Low | $32 | $52 |
|
IDT Human Exome (batches of 12 samples) |
LP-cap-hum-ex |
$241 |
$389 |
| IDT Human Exome (batches of 12 samples) Tier 2: Price/sample 48-N |
LP-cap-hum-ex | $129 | $209 |
| FFPE - DNA-Seq library with KAPA HyperPrep per specimen Tier 1: Price/sample 1-47 |
LP-FFPE-DNA-Seq | $206 | $332 |
| FFPE - DNA-Seq library with KAPA HyperPrep per specimen Tier 2: Price/sample 48-N |
LP-FFPE-DNA-Seq | $163 | $263 |
|
ChIP-seq/amplicon Library Preparation with KAPA HyperPrep per sample |
LP-DNA-ChIP-amp |
$86 |
$140 |
| ChIP-Seq/amplicon library with KAPA HyperPrep per specimen Tier 2: Price/sample 48-N |
LP-DNA-ChIP-amp | $65 | $79 |
|
Custom Capture Panels |
Contact us |
|
|
RNA-Sequencing Illumina Library Building Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
mRNA-Seq library with KAPA HyperPrep per sample |
LP-RNA-Seq
|
$95 |
$154 |
| mRNA-Seq library with Human Globin AnyDeplete per sample | LP-RNA-Seq-glob | $155 | $251 |
| Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample | LP-RNA-total | $151 | $189 |
|
Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample |
LP-RNA-total
|
$143 |
$170 |
|
smRNA-Seq library per sample |
LP-RNA-sm-Seq |
$156 |
$252 |
|
Takara ultra-low-input RNA-Seq library with KAPA HyperPrep per sample |
LP-RNA-Seq-lo-B |
$173 |
$280 |
|
FFPE - Total RNA library with Ribo-Zero Plus per sample |
LP-FFPE-RNA-seq |
$280 |
$451 |
| FFPE - Total RNA library with Ribo-Zero Plus per sample Tier 2: Price/sample 48-N |
LP-FFPE-RNA-seq | $191 | $313 |
Illumina Sequencing Services
| Platform | Flow Cell | Read Length** | Code | Duke Price | Non-Duke Price |
|---|---|---|---|---|---|
| MiSeq | v2 NANO | 2 x 150 | I-Mi-v2-n-150 | $808 | $1,088 |
| v2 NANO | 2 x 250 | I-Mi-v2-n-250 | $886 | $1,169 | |
| v2 MICRO | 2 x 150 | I-Mi-v2-µ-150 | $1,004 | $1,293 | |
| v2 | 1 x 50 | I-Mi-v2-050-SR | $1,500 | $1,814 | |
| v2 | 2 x 150 | I-Mi-v2-150-SR | $1,802 | $2,131 | |
| v2 | 2 x 250 | I-Mi-v2-250-SR | $1,973 | $2.311 | |
| v3 | 2 x 75 | I-Mi-v3-075 | $1,619 | $1,939 | |
| v3 | 2 x 300 | I-Mi-v3-075 | $2,439 | $2,800 | |
| NextSeq 1000 | XLEAP P1 | 2 x 50 | I-NEXT-P1-X-100 | $1,227 | $1,533 |
| XLEAP P1 | 2 x 150 | I-NEXT-P1-X-300 | $1,535 | $1,862 | |
| XLEAP P1 | 2 x 300 | I-NEXT-P1-X-600 | $2,107 | $2,457 | |
| XLEAP P2 | 2 x 50 | I-NEXT-P2-X-100 | $1,637 | $1,964 | |
| XLEAP P2 | 2 x 100 | I-NEXT-P2-X-200 | $2,694 | $3,073 | |
| XLEAP P2 | 2 x 150 | I-NEXT-P2-X-300 | $3,430 | $3,846 | |
| XLEAP P2 | 2 x 300 | I-NEXT-P2-X-600 | $3,695 | $4,124 | |
| NextSeq 1000 | P1 | 2 x 50 | I-Next-P1-050 | $1,282 | $ 1,567 |
| P1 | 2 x 100 | I-Next-P1-100 | $1,742 | $2,074 | |
| P1 | 2 x 150 | I-Next-P1-150 | $1,742 | $2,074 | |
| P1 | 2 x 300 | I-Next-P1-300 | $2,421 | $2,787 | |
| P2 | 2 x 50 | I-Next-P2-050 | $1,956 | $2,299 | |
| P2 | 2 x 100 | I-Next-P2-100 | $3,295 | $3,704 | |
| P2 | 2 x 150 | I-Next-P2-150 | $4,227 | $4,683 | |
| NovaSeq X Plus | 1.5B | 2 x 50 | I-X-1.5B-100 | $2,589 | $2,999 |
| 1.5B | 2 x 100 | I-X-1.5B-200 | $3,139 | $13,576 | |
| 1.5B | 2 x 150 | I-X-1.5B-300 | $3,359 | $3,807 | |
| 1.5B | Any Configuration*** | I-X-1.5B-lane | $1,929 | $2,306 | |
| 10B | 2 x 50 | I-X-10B-100 | $10,798 | $13,012 | |
| 10B | 2 x 100 | I-X-10B-200 | $12,888 | $15,206 | |
| 10B | 2 x 150 | I-X-10B-300 | $13,548 | $15,899 | |
| 10B | Any Configuration*** | I-X-10B-Lane | $1,8181 | $2,188 | |
| 25B | 2 x 150 | I-X-25B-300 | $22,832 | $26,904 | |
| 25B | Any Configuration*** | I-X-25B-lane | $2,872 | $3,504 |
**default sequencing protocol; we can run custom sequencing protocols with purchase of full flow cell
***We can run custom read length protocols on NovaSeq X Plus lanes. Custom primers must be run on a full flow cell
PacBio Library Building and Sequencing
| Description | Code | Duke Price | Non-Duke Price |
|---|---|---|---|
| HiFi library (large genomes > 200 MB) per sample | PB-LP-HiFi | $568 | $916 |
| HiFi library (small genomes < 200 MB) per sample | PB-LP-HiFi-2 | $157 | $254 |
| Kinnex 16S library per sample | PB-LP-Kin-16S | $946 | $1,524 |
| Kinnex single cell RNA library per sample | PB-LP-MAS | $946 | $1,524 |
| RNA library per sample | PB-LP-RNA | $683 | $1,101 |
| Revio SMRT cell 30 -hour sequencing | PB-Rev-SMRT-30 | $1,783 | $2,396 |
Extraction and Other Wet-Lab Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
DNA extraction from PBMCs, buffy coat blood, or EDTA blood per specimen |
SP-DNA-blood |
$45 |
$73 |
|
HMW DNA extraction per specimen |
SP-DNA-HMW |
$72 |
$117 |
|
RNA extraction from blood per specimen |
SP-RNA-blood |
$44 |
$72 |
|
RNA extraction from cell pellet per specimen |
SP-RNA-cell |
$41 |
$67 |
|
miRNA extraction from serum or plasma per specimen |
SP-miRNA-serum |
$41 |
$67 |
|
Quantification (qPCR) per library |
LP-X-qPCR |
$45 |
$73.50 |
| AMPure bead cleanup per library | LP-X-rm-ad-dim | $46 | $75 |
|
Pooling for up to 12 client-made libraries |
Pooling |
$183 |
$220 |
While we no longer offer QC only services, the Microbiome Core Facility does.
Bioinformatics***
| Description | Code | Duke Price |
|---|---|---|
| Free One-Hour Bioinformatics Consultation - over $450 value | B-free-consult | $0 |
| Free One-Hour Bioinformatics Wrap-Up Meeting - Over $300 value | B-free-wrap-up | $0 |
| Whole Exome Pipeline per Sample | B-exome | $207 (sample #1-4); $10/additional sample |
| Whole Genome Pipeline per Sample | B-genome |
$207 (sample #1-4); $10/additional sample |
| RNA-Seq Pipeline per Sample | B-RNA-Seq | $104 (sample #1-6); $10/additional sample |
| ATAC-Seq/ChIP-Seq Pipeline per Sample | B-ATAC-ChIP-Seq | $227 (sample #1-3); $10/additional sample |
| Methyl-Seq Pipeline per Sample |
B-Methyl-Seq |
$227 (sample #1-4); $10/additional sample |
| Mapping and Basic QC per Sample | B-mapping-QC | $161 (sample #1-6); $5/additional sample |
| Hourly Rate | B-rate-60 | $175 |
| Quarter-Hourly Rat | B-rate-15 | $43.75 |
|
SoM CPU charges |
B-CPU | $0.02 |
***Not available outside of Duke