*Prices as of February 23, 2026. Pricing is subject to change. Please visit SeqLIMS for up-to-date pricing. If you don’t see your library preparation of interest, please reach out to us. External academic clients may provide their own reagents for PacBio sequencing and can reach out to us to inquire about pricing and logistics. Potential industry partners can reach out to us to inquire about pricing.
We are a cost-recovery shared resource and do not make a profit on our services per Duke policy. Our price includes our facility fee for our services and the cost of consumables. Per Duke policy, there are no discounts on services.
- DNA-Sequencing Illumina Library Building Services
- RNA-Sequencing Illumina Library Building Services
- Illumina Sequencing Services
- PacBio Library Building and Sequencing
- Extraction and Other Wet-Lab Services
- Bioinformatics Services
DNA-Sequencing Illumina Library Building Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
DNA-Seq with Watchmaker per sample |
LP-DNA-WM-enz
|
$71.45 |
$98.01 |
| DNA-Seq with Watchmaker per sample Samples 49-N |
LP-DNA-WM-enz | $50.95 | $65.01 |
|
Tagmentation library - full volume reaction for low DNA input |
LP-DNA-TAG-01
|
$89.40 |
$116.83 |
|
Tagmentation library - full volume reaction for low DNA input |
LP-DNA-TAG-01 |
$68.90 |
$83.83 |
| Tagmentation library - 1/4 volume reaction for high DNA input Sample 1-48 |
LP-DNA-TAG-Low | $58.05 | $83.91 |
| Tagmentation library - 1/4 volume reaction for high DNA input Samples 49-N |
LP-DNA-TAG-Low | $37.55 | $50.91 |
|
Whole Exome Sequencing Human - TWIST (batches of 8) |
LP-cap-hum-ex |
$1,596.80 |
$2,047.28 |
| Whole Exome Sequencing Human - TWIST (batches of 8) Bathes 7-N |
LP-cap-hum-ex | $1,328.25 | $2,176.02 |
| FFPE - DNA-Seq library prep with Watchmaker per sample Samples 1-48 |
LP-FFPE-DNA-Seq | $153.45 | $228.48 |
| FFPE - DNA-Seq library prep with Watchmaker per sample Sample 49-N |
LP-FFPE-DNA-Seq | $112.45 | $162.47 |
|
ChIP-seq/amplicon library prep with Watchmaker per sample |
LP-DNA-WM-lig |
$71.45 |
$98.01 |
| ChIP-Seq/amplicon library prep with Watchmaker per sample Sample 49-N |
LP-DNA-WM-lig | $50.95 | $65.01 |
| Enzymatic Methylation-Sequencing per sample | LP-DNA-EM-Seq | $89.50 | $116.94 |
|
Custom Library Builds per sample |
LP-custom |
$164.00 |
$264.04 |
RNA-Sequencing Illumina Library Building Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
| mRNA sequencing < 24 samples 50M/reads per sample | LP-mRNA-bulk | $159 | $197.78 |
|
mRNA sequencing library preparation samples 24-n |
LP-mRNA-24
|
$88.30 |
$115.68 |
|
Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample |
LP-RNA-total | $151.42 | $181.95 |
|
Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample |
LP-RNA-total | $130.92 | $148.95 |
|
mRNA seq library with Human Globin |
LP-RNA-Seq-glob
|
$171.48 |
$214.50 |
|
smRNA-Seq library |
LP-RNA-sm-Seq |
$152.21 |
$182.78 |
|
Takara ultra-low-input RNA-Seq library with Watchmaker |
LP-RNA-Seq-lo-B |
$183.54 |
$238.64 |
|
FFPE - Total RNA library with Ribo-Zero Plus (human, mouse, rat) per sample |
LP-FFPE-RNA-seq |
$274.42 |
$379.98 |
| FFPE - Total RNA library with Ribo-Zero Plus (human, mouse, rat) Sample 49-N |
LP-FFPE-RNA-seq | $192.42 | $247.96 |
Illumina Sequencing Services
| Platform | Flow Cell | Read Length** | Code | Duke Price | Non-Duke Price |
|---|---|---|---|---|---|
| MiSeq i100 | 100M | 300 Cycles (150x150) |
I-Mi-100M-300 | $2,071.75 | $2.399.65 |
| 25M | 100 Cycles (50x50) |
I-Mi-25M-100 | $1,032.25 | $1,306.18 | |
| 25M | 300 Cycles (150 x 150) |
I-Mi-25M-300 | $1,436.50 | $1,730.65 | |
| 25M | 600 Cycles (300x300) |
I-Mi-25M-600 | $1,667.50 | $1,973.20 | |
| 25M | 1000 Cycles (500x500) |
I-Mi-25M-1000 | $2,333.50 | $2,700.85 | |
| 50M | 100 Cycles (50x50) |
I-Mi-50M-100 | $1,263.25 | $1,550.72 | |
| 50M |
300 Cycles |
I-Mi-50M-300 | $1,667.50 | $1,975.19 | |
| 50M | 600 Cycles (300 x 300) |
I-Mi-50M-600 | $2,071.75 | $2399.65 | |
| 5M | 300 Cycles (150x150) |
I-Mi-5M-300 | $882.10 | $1,148.53 | |
| 5M | 600 Cycles (300 x 300) |
I-Mi-5M-600 | $1,147.75` | $1,427.46 | |
| NextSeq 1000/2000 | XLEAP P1 | 100 Cycles (50x50) |
I-NEXT-P1-X-100 | $1,229 | $1,535.17 |
| XLEAP P1 | 300 Cycles (150x150) |
I-NEXT-P1-X-300 | $1,537 | $1,858.57 | |
| XLEAP P1 | 600 Cycles (300x300) |
I-NEXT-P1-X-600 | $2,109 | $2,459.17 | |
| XLEAP P2 | 100 Cycles (50x50) |
I-NEXT-P2-X-100 | $1,638.20 | $1,964.83 | |
| XLEAP P2 | 200 Cycles (100x100) |
I-NEXT-P2-X-200 | $2,695.30 | $3,074.79 | |
| XLEAP P2 | 300 Cycles (150x150) |
I-NEXT-P2-X-300 | $3,431.20 | $3,847.48 | |
| XLEAP P2 | 600 Cycles (300x300) |
I-NEXT-P2-X-600 | $3,696.30 | $4,125.84 | |
| XLEAP P3 | 100 Cycles (50x50) |
I-NEXT-P3-X-100 | $3,002.20 | $3,3397.57 | |
| XLEAP P3 | 200 Cycles (100x100) |
I-NEXT-P3-X-200 | $3,988.90 | $4,433.57 | |
| XLEAP P3 | 300 Cycles (150x150) |
I-NEXT-P3-X-300 | $5,172.50 | $5,676.57 | |
| XLEAP P4 | 100 Cycles (50x50) |
I-NEXT-P4-X-100 | $3,407 | $3,822.57 | |
| XLEAP P4 | 200 Cycles (100x100) |
I-NEXT-P4-X-200 | $4,636.80 | $5,113.57 | |
| XLEAP P4 | 300 Cycles (150x150) |
I-NEXT-P4-X-300 | $5,783 | $6,317.57 | |
| NovaSeq X Plus | 1.5B | 100 Cycles (50x50) |
I-X-1.5B-100 | $3.244.70 | $4,018.46 |
| 1.5B | 200 Cycles (100x100) |
I-X-1.5B-200 | $3,811.20 | $4,613.28 | |
| 1.5B | 300 Cycles (150x150) |
I-X-1.5B-300 | $4,037.80 | $4,851.21 | |
| 1.5B | Any Configuration*** | I-X-1.5B-lane | $2,018.90 | $2,425.61 | |
| 10B | 100 Cycles (50x50) |
I-X-10B-100 | $10,694.40 | $12,976.32 | |
| 10B | 200 Cycles (100x100) |
I-X-10B-200 | $12,664.96 | $15,045.41 | |
| 10B | 300 Cycles (150x150) |
I-X-10B-300 | $13,392.80 | $15,809.64 | |
| 10B | Any Configuration*** | I-X-10B-Lane | $1,709.73 | $2,086.41 | |
| 25B | 100 Cycles (50x50) |
I-X-25B-100 | $17,053.50 | $19,888.58 | |
| 25B | 200 Cycles (100x100) |
I-X-25B-200 | $20,287.50 | $23,284.20 | |
| 25B | 300 Cycles (150x150) |
I-X-25B-300 | $22,574.40 | $25,685.52 | |
| 25B | Any Configuration*** | I-X-25B-lane | $2,969.30 | $3,448.17 |
**default sequencing protocol; we can run custom sequencing protocols with purchase of full flow cell
***We can run custom read length protocols on NovaSeq X Plus lanes. Custom primers must be run on a full flow cell
PacBio Library Building and Sequencing
| Description | Code | Duke Price | Non-Duke Price |
|---|---|---|---|
| IsoSeq prepared cDNA from Total RNA | PB_LP_cDNA | $262.22 | $325.59 |
| HiFi library (large genomes > 200 MB) per sample | PB-LP-HiFi | $590.58 | $821.15 |
| HiFi library (small genomes < 200 MB) per sample | PB-LP-HiFi-2 | $160.71 | $219.01 |
| Kinnex 16S library per sample | PB-LP-Kin-16S | $945.40 | $1,193.71 |
| Single cell RNA library per specimen | PB-LP-MAS | $953.14 | $1,201.84 |
| RNA library per sample | PB-LP-RNA | $953.14 | $1,201.84 |
| Revio SMRT cell 30 -hour sequencing | PB-Rev-SMRT-30 | $1,960.94 | $2,536.11 |
Extraction and Other Wet-Lab Services
|
Description |
Code |
Duke Price |
Non-Duke Price |
|---|---|---|---|
|
DNA extraction from PBMCs, buffy coat blood, or EDTA blood per specimen |
SP-DNA-blood |
$47.86 |
$68.43 |
|
HMW DNA extraction per specimen |
SP-DNA-HMW |
$87.75 |
$110.31 |
| miRNA extraction from serum or plasma per specimen | SP-miRNA-serum | $46.05 | $66.52 |
|
RNA extraction from blood per specimen |
SP-RNA-blood |
$47.75 |
$68.31 |
|
RNA extraction from cell pellet per specimen |
SP-RNA-cell |
$45.78 |
$66.25 |
|
Quantification (qPCR) per library |
LP-X-qPCR |
$42.55 |
$67.64 |
| AMPure bead cleanup per library | LP-X-rm-ad-dim | $44.03 | $69.19 |
| Additional advanced QC of DNA or library via Qubit + Femto Pulse per sample | SP-X-DNA-QC-adv | $71 | $97.51 |
| Additional basic QC of DNA via Qubit per sample | SP-X-DNA-QC-bas | $42.16 | $67.22 |
| Additional QC of RNA per sample | SP-X-RNA-QC | $44.30 | $69.48 |
|
Pooling for up to 12 libraries |
Pooling |
$190 |
$275.10 |
| Replating Charge | SP-replate | $5 | $8.05 |
| NextSeq/MiSeq custom primer | I-X-primer | $100 | $105 |
| Wire transfer | X-wire-transfer | $31 |
While we no longer offer QC only services, the Microbiome Core Facility does.
Bioinformatics***
| Description | Code | Duke Price | External (Non-profit) |
|---|---|---|---|
| Free One-Hour Bioinformatics Consultation - over $450 value | B-free-consult | $0 | |
| Free One-Hour Bioinformatics Wrap-Up Meeting - Over $300 value | B-free-wrap-up | $0 | |
|
ATAC-Seq/ChIP-Seq pipeline |
B-ATAC-ChIP-Seq | $246.00 | |
| ATAC-Seq/ChIP-Seq pipeline Samples 14-N |
B-ATAC-ChIP-Seq | $12.50 | |
| Whole Exome Pipeline Samples 1-7 |
B-exome | $224 | |
| Whole Exome Pipeline Samples 8-N |
B-exome | $11.50 | |
| Whole Genome Pipeline Samples 1-6 |
B-genome |
$224 |
|
| Whole Genome Pipeline Samples 7-N |
B-genome | $11.50 | |
| Methyl-Seq pipeline Samples 1-13 |
B-Methyl-Seq | $246 | |
| Methyl-Seq pipeline Samples 14-N |
B-Methyl-Seq | $12.50 | |
| RNA-Seq Pipeline Samples 1-12 |
B-RNA-Seq | $110 | |
| RNA-Seq Pipeline Samples 13-N |
B-RNA-Seq | $11 | |
| Mapping and Basic QC | B-mapping-QC | $165 | |
| Quarter-Hourly Rate | B-rate-15 | $43.25 | |
| Hourly Rate | B-rate-60 | $173 | $278.53 |
|
Download from deep storage |
B-X-download | $0.10 | $0.17 |
***Not available outside of Duke