Proteomics and Metabolomics Pricing

Pricing For Core Service Support for Duke and Non-Duke Scientists

Note: Scientific collaborations with academic, government, and non-profit laboratories outside Duke are welcome. Contact us for details. 

Please note: Prices below are based on an a-la-carte menu of services to maximize flexibility in experimental designs and comply with auditing regulations. Most analyses will require combinations of these services. Please see the FAQs for additional information. All prices are effective July 1, 2023.

Sample Preparation (Proteomics)

  Duke Non-Duke
Mini-Bradford Assay (per plate, 20 samples max) $47 $76
   - NOTE - Bradford Assays required for all solution phase analyses    
General Sample Preparation - Proteomics (cost per method) $61 $99
           In-Solution Digestion*    
            FFPE Protein Extraction    
           In-situ/bead-bound digestion    
Tissue Disruption/Lysis and Protein Extraction (RapiGest, Urea,TriZol, or Bead Blasting)        $27 $44
In-Gel Digestion (per spot or band)* $64 $103
S-trap Digestion $95 $153
Sample Cleanup Procedures (cost per method) $49 $79
            Solid Phase Extraction (C18, HLB, MCX)    
            Buffer exchange by ZebaSpin    
            Protein Precipitation and Resuspension    
            Biotin-Avidin enrichment    
Amicon MW Cutoff Filtration $64 $103
TiO2 enrichment for phosphopeptides $60 $97
1D PAGE Gel Separation (Protein Level) $140 $226
Offline Fractionation (high pH C18) $219 $353
Antibody-Based Enrichment (not including antibody reagent costs) $78 $125
*Additional reagents charge for digestions with enzymes other than Trypsin* Contact Contact


Sample Preparation (Metabolomics)

  Duke Non-Duke
MeOH Precipitation with Internal Standards - Biofluids included included
Solid Sample Solubilization (Cells, Tumor) by Bead Blasting, Probe Sonication, or Tissue Tearing $27 $44
Mini-Bradford Assay (per plate, 20 samples max) $47 $76
  -NOTE - this is used to normalize to total biomass    
Specialized Per-Sample Preparation for Specific Assays is Included in the Cost/Sample n/a n/a
Pharmacokinetics Plate Prep (biofluids) $78 $125


Qualitative Analyses (Proteomics)

  Duke Non-Duke
LC-MS/MS analysis (simple in-gel digest - very fast gradient) $104 $167
LC-MS/MS analysis (in-gel or solution digest of simple sample - fast gradient) $162 $261
LC-MS/MS analysis (moderately complex - medium gradient) $213 $342
LC-MS/MS analysis (complex sample - slow gradient) $253 $407
Molecular Weight Determination (per sample) $77 $125
         MW Setup Fee (per order) $177 $284
ZipChip Profiling Method $77 $124

Quantitative Analyses, Unbiased (Proteomics)

  Duke Non-Duke
LC-MS/MS Differential Expression (per sample), 15-min. gradient $108 $174
LC-MS/MS Differential Expression (per sample), 30-min. gradient $224 $361
LC-MS/MS Differential Expression (per sample), 60-min. gradient $285 $459
*LC-MS/MS Differential Expression (per sample), 90-min. gradient $345 $556
Quantitative 1D Workup - Proteome Discoverer, Spectronaut or Elucidator (analysis per sample) $69 $111


Quantitative Analyses, Targeted (Proteomics)

*Stable Isotope Labeled Peptides are provided at third party vendor cost. Methods are developed and performed on nanoAcquity UPLC and TQ-S triple quadrupole mass spectrometer unless otherwise stated. The shortest method possible is used for quantification of the number of analytes requested, using retention-time scheduled MRM technology. Typically, at least two peptides per protein and three transitions per peptide are used for MRM quantitative assays.

  Duke Non-Duke
Targeted (MRM) Quantitation Method Development, priced per hour Contact Contact
MRM Quantitative Data Collection (30 min gradient) $151 $244
MRM Quantitative Data Collection (60 min gradient) $212 $341
MRM Quantitative Data Collection (90 min gradient) $242 $390
MRM Data Analysis in Skyline, priced per hour Contact Contact


Quantitative Analysis (Metabolomics)

All prices are on a per-sample basis.

  Duke Non-Duke
Acylcarnitines analysis** $24 $39
AbsoluteIDQ p180 Panel (kit from Biocrates, Inc.)* $60 $97
MxP Quant 500 Panel (kit from Biocrates, Inc.)* $85 $138
Bile Acids Panel (kit from Biocrates, Inc.)* $43 $69
Oxylipins/Eicosanoid Panel** $156 $251
Methionine Pathway Panel** $94 $151
Hydroxycholesterols Panel** $136 $219
27-Hydroxycholesterol quantitation** $88 $142
Fatty Acids Panel** $80 $129
Fast HR Metabolite Profiling (RPLC OR HILIC, ESI+ OR ESI-) $172


High Resolution Lipid Profiling, ESI+ and ESI- $306 $493
PK Analysis - method development Contact Contact
     per sample with in-house developed method** $35 $56
Short Chain Fatty Acids Analysis** $76 $123
Sphingosine S1P Panel** $53 $86
ZipChip Profiling Method $77 $124
Neurotransmitter Panel** $80 $129
Nucleosides** $61 $98

*These assays require additional purchase of kit consumables from Biocrates, Inc. Negotiated prices may vary significantly depending on the number of samples to be analyzed.

**These assays will incur an extra charge for running a standard curve, quality control injections, and blanks.


Data Analysis

  Duke Non-Duke
Custom Database Setup for MASCOT or Identify $26 $43
Additional Database Searches $34 $55
Consulting and Extensive Data Mining (per hour) $69 $111

For additional pricing examples for your particular study, contact us.