My research focuses on the regulation of genetic stability and primarily uses budding yeast (Saccharomyces cerevisiae) as a model genetic system. The two primary research goals in the budding yeast system are (1) defining molecular structures and mechanisms of mitotic recombination intermediates and (2) understanding how and why transcription destabilizes the underlying DNA template. We also have initiated studies of mutagenesis in the pathogenic fungus Cryptococcus neoformans. We have found that a shift to the human body temperature mobilizes transposable elements, and suggest that this promotes rapid adaptation to the harsh host environment.
Education and Training
- University of Wisconsin at Madison, Ph.D. 1983
Selected Grants and Awards
- Regulation of mitotic genome stability in yeast.
- Genetics Training Grant
- Organization and Function of Cellular Structure
- Molecular Mycology and Pathogenesis Training Program
- Temperature-dependent transposon mobilization in Cryptococcus neoformans
- Non-Canonical Responses to DNA damage in Drosophila Polyploid Cells
- Investigating the origin of spontaneous mitotic homologous recombination in Saccharomyces cerevisiae
- Regulation of mitotic genome stability in yeast
- Topoisomerase 1 and mutagenesis in yeast
- Transcription-associated mutagenesis in yeast
- Tolerance of spontaneous and induced DNA in yeast
- Role of cross-link formation in Pol Zeta-dependent mutagenesis in S. cerevisiae