Biophysical analysis of coreceptor modulation of TCR-MHC interactions. One of our research interests is to study the molecular mechanisms of T cell recognition. We have particular interest in understanding the trimolecular interactions between membrane bound T cell receptor (TCR-CD3 complex) and its ligand, the peptide-MHC complex (pMHC), and co-receptor molecules. We are using different biophysical approaches which include surface plasmon resonance, isothermal titration calorimetry and atomic force spectroscopy to study the influence of co-stimulatory molecules on the kinetics and thermodynamics of TCR-pMHC binding. The goal is to use integrated molecular and biophysical approaches to study membrane TCR-MHC interactions and to understand how these interactions are modulated by co-receptors during T cell development and activation.
Energetics and kinetics of monoclonal antibody binding to soluble HIV Envelope proteins. An additional interest of our lab involves biophysical characterization of soluble, recombinant human HIV-1 Envelope proteins (gp120 and gp140). We have employed surface plasmon resonance, analytical sedimentation equilibrium analysis, isothermal titration calorimetry and atomic force spectroscopy to characterize the antigenicity and study the energetics, kinetics and single molecule force measurements of the binding of anti-gp120 and anti-gp41 antibodies to several recombinant HIV-1 Envelope proteins. These studies have relevance in immunogen design for generating anti-HIV vaccines.
Education and Training
- University of Glasgow (Scotland), Ph.D. 1992
Selected Grants and Awards
- Innate antiviral factors in breast milk and the oral HIV-1 reservoir
- Aptamers as Proteomic Tools for Pancreatic Cancer Biomarker Identification
- University Training Program in Biomolecular and Tissue Engineering
- Nonhuman Primate Core-Option 4
- Permar IMPAACT 2004 stability study QAU
- Multiscale Systems Immunology
- HIV-1 Neutralizing Antibody Binding to Viral Membrane Mimics
- MPER broadly neutralizing antibody knockin mice to study anti-HIV Bcell responses
- Multiscale Integrative Immunology for Adjuvant Develpmnt
- Modulation of CD8 T-Cells
- BIACORE 3000 Biosensor