My laboratory is interested in fungal genomics.
In particular we use genomic sequencing of fungal strains and species in comparative analysis. Starting with the sequencing of Saccharomyces cerevisiae strain S288C, I have been involved in the genome sequencing and annotation of Ashbya gossypii, Cryptococcus neoformans var. grubii and ~100 additional S. cerevisiae strains. We currently use Illumina paired end and mate paired sequencing, as this is at presently the most cost effective widely used technology capable of generating high accuracy, zero gap whole genome sequences. The 100-genomes S. cerevisiae data as well as the fully updated fully annotated A. gossypii sequence (Genbank numbers AE016814-AE016820), which spans all seven chromosomes from telomere to telomere, were generated using Illumina data. In my laboratory we strive to utilize comparative genomics data to understand aspects of basic fungal biology. Some of our specific areas of interest are filamentous growth, mapping of complex traits, horizontal gene transfer, and identification of RNA coding genes. This work involves a combination of experimental work and bioinformatics analysis. Research in S. cerevisiae has greatly benefitted from an accurate, annotated S. cerevisiae reference genome, and that research into the tremendous diversity in this organism will similarly benefit from the availability of a large number of accurate, fully annotated genome sequences. The use of genomic information to better understand the biology of these organisms, and this is what students in my laboratory generally work on.
What is the set of genes found in a pathogenic fungus such as Cryptococcus?
Our interest in this human pathogen is to expand beyond looking at one isolate and to investigate the diversity in the population. Are there genes found in some Cryptococcus neoformans isolates but not in others? Are there regions of the genome or individual genes which are highly diverged between Cryptococcus isolates? Efforts are now underway at Stanford University to sequence the genome of the JEC21 strain of Cryptococcus. This is a strain that has been agreed upon by the community of Cryptococcus researchers as a reference strain. Obtaining the DNA sequence of this strain is only the start however. From that sequence identifying the complete set of genes will be a considerable challenge requiring both bioinformatic as well as experimental tools. While this work on gene identification is going on we plan on addressing the question of how much do other Cryptococcus isolates differ from JEC21.
What is the set of genes in humans?
The complete DNA sequence of human and mouse will become available soon. This does not mean that we will know the complete set of human or mouse genes. Our current state of knowledge does not allow us to accurately predict human genes directly from DNA sequence. We are interested in applying to the human genome some of the experimental and bioinformatic tools we are developing and utilizing in fungal systems.
Education and Training
- University of California at Davis, B.S. 1985
- Massachusetts Institute of Technology, Ph.D. 1996
- Massachusetts Institute of Technology, Research Associate, Biology
- Stanford University, Head, DNA Sequencing Center, Dna Sequencing Ctr
Selected Grants and Awards
- Transplant Infectious Diseases Interdisciplinary Research Training Grant
- Summer Scholars Program in Genome Sciences and Medicine
- Bioinformatics and Computational Biology Training Program
- Genetics of Cryptococcus sexual reproduction
- Genetics Training Grant
- Organization and Function of Cellular Structure
- Molecular Mycology and Pathogenesis Training Program
- Pathobiology of C. neoformans in the Central Nervous System
- High throughput S. cerevisiae HAM, GWA & QT/QTL architecture
- Evolution of Cryptococcus neoformans strains from patients with HIV/AIDS
- Evolution of silencing proteins in yeast
- Genetic Analysis in Chlamydia
- FIBR: Integrated Ecological and Genomic Analysis of Speciation in Mimulus
- Genetic analysis of Cryptococcus neoformans virulence
- A Genomics Approach to Study C. neoformans var. grubii
- Duke/NIDDK Functional Genomics Center